Difference between revisions of "Part:BBa K2804005"

Revision as of 01:07, 18 October 2018

CBD cipA fused to sfGFP under the control of LacI promoter

The superfolder green fluorescent protein (sfGFP) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 230
Illegal AgeI site found at 1493
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+ </div>
+				</div>
+				<div class="ec--BMP--container">
+					<div class="ec--h3"><a name="SF">CBD cipA-sfGFP</a></div>
+				  <div class="ec--BMP--img" style="float: left; display: block; text-indent: inherit; width: 288px; text-align: justify; margin-right: 20px;margin-bottom: 20px"><center>
+                    <p><img src="https://static.igem.org/mediawiki/2018/e/e4/T--Ecuador--CBD-sfGFP.png" width="268" height="261"/></p>
+                      <p><strong>Figure 2. </strong>Tridimensional structure of CBD cipA-sfGFP  obtained from the server I-Tasser.</p>
+				  </center></div><div class="ec--p">
+						  <p>The gBlocks synthetized for in-frame protein assembly corresponded to the first gBlock consisting of lacI promoter, RBS and CDS of CBD cipA; the second gBlock containing the CDS of sfGFP with a 6X His tag. Both of them were codon optimized for <em>E</em>. <em>coli</em>.</p>
+						  <p>We aimed to add a 6X His tag at C-terminus in order to visualize and measure at single-molecule level the interaction between the CBD cipA and bacterial cellulose.<sup>6</sup></p>
+						<p><center><img src="https://static.igem.org/mediawiki/2018/5/5b/T--Ecuador--CBD_cipA-sfGFP_construct_Map.png" width="450" height="450"/></center></p><br>
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>