Difference between revisions of "Part:BBa K2565001"
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<partinfo>BBa_K2565001 short</partinfo> | <partinfo>BBa_K2565001 short</partinfo> | ||
| − | This composite part is used to constitutively express high levels of Shewanella oneidensis MR-1 fermentative lactate dehydrogenase NADH dependent | + | <p>This composite part is used to constitutively express high levels of <i>Shewanella oneidensis</i> MR-1 fermentative lactate dehydrogenase NADH dependent <i>ldhA</i> (BBa_K2565000) in <i>E. coli</i>, locus tag SO_0968. </p> |
| − | + | <p><img src="https://static.igem.org/mediawiki/2018/d/d3/T--Pittsburgh_CSL--pic2a.jpeg" width="400" height="300" > | |
| + | <img src="https://static.igem.org/mediawiki/parts/5/54/T--Pittsburgh_CSL--results3.jpeg" width="400" height="300" ></p> | ||
| + | <p>The left figure shows that the media with <i>E. coli</i> constitutively expressing <i>ldhA</i> contains more lactate than LB only. The right figure shows the amount of lactate in the media of cultures so that when <i>Shewanella</i> and <i>E. coli</i> are grown together there is more lactate in the media. The MG1655 strain of <i>E. coli</i> does not express the <i>Shewanella ldhA</i> gene.</p> | ||
| − | |||
| − | https://static.igem.org/mediawiki/parts/ | + | <p><img src="https://static.igem.org/mediawiki/parts/6/62/T--Pittsburgh_CSL--results.jpeg" width="400" height="300" ></p> |
| + | |||
| + | <p>This graph shows the voltage produced by microbial fuel cells (MFC) in 15 hours. The MFC with <i>Shewanella</i> and <i>E. coli</i> produced more voltage than <i>Shewanella</i> alone. The MFC with <i>Shewanella</i> and <i>E. coli</i> expressing <i> Shewanella ldhA</i> produced more consistent voltage than <i>E. coli</i> without <i> Shewanella ldhA</i>.</p> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 01:04, 18 October 2018
Shewanella ldhA gene highly expressed in E. coli
This composite part is used to constitutively express high levels of Shewanella oneidensis MR-1 fermentative lactate dehydrogenase NADH dependent ldhA (BBa_K2565000) in E. coli, locus tag SO_0968.
<img src="
" width="400" height="300" >
<img src="
" width="400" height="300" >
The left figure shows that the media with E. coli constitutively expressing ldhA contains more lactate than LB only. The right figure shows the amount of lactate in the media of cultures so that when Shewanella and E. coli are grown together there is more lactate in the media. The MG1655 strain of E. coli does not express the Shewanella ldhA gene.
<img src="
" width="400" height="300" >
This graph shows the voltage produced by microbial fuel cells (MFC) in 15 hours. The MFC with Shewanella and E. coli produced more voltage than Shewanella alone. The MFC with Shewanella and E. coli expressing Shewanella ldhA produced more consistent voltage than E. coli without Shewanella ldhA.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]