Difference between revisions of "Part:BBa K2588017"

 
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<p>We designed ADH2 as a part of the lure module to make <i>E. coli</i> produce 3-methyl-1-butanole to attract mosquitos. ADH2 as well as <a href="https://parts.igem.org/Part:BBa_K2588016">kivD</a> is needed to convert 2-ketoisocaproate into 3-methyl-1-butanole.<sup>1</sup></p>
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<h2>Usage and Biology</h2>
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<p>ADH2 is the glucose-repressible alcohol dehydrogenase 2. It naturally occurs in <i>Saccharomyces cerevisiae</i>. It is NAD-dependent and normally converts ethanol into acetylaldehyde. But it can act on a variety of primary unbranched aliphatic alcohol such as 2-ketoisocaproate. We expressed ADH2 in E. coli to use it as part of our Lure Module. Together with kivD it converts 2-ketoisocaproate, which was prepared by LeuABCD, into 3-methyl-1-butanole.<sup>2</sup></p>
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<h2>References</h2>
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<li>Yeastgenome.org. (2018). ADH2 | SGD. [online] Available at: https://www.yeastgenome.org/locus/S000004918 [Accessed 18 Oct. 2018
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<li>Connor, M. R., Cann, A. F. & Liao, J. C. 3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation. <i>Appl. Microbiol. Biotechnol.</i> <b>86</b>, 1155–1164 (2010).</li>
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Revision as of 01:01, 18 October 2018


Alcohol dehydrogenase 2 from Saccharomyces cerevisiae

We designed ADH2 as a part of the lure module to make E. coli produce 3-methyl-1-butanole to attract mosquitos. ADH2 as well as kivD is needed to convert 2-ketoisocaproate into 3-methyl-1-butanole.1

Usage and Biology

ADH2 is the glucose-repressible alcohol dehydrogenase 2. It naturally occurs in Saccharomyces cerevisiae. It is NAD-dependent and normally converts ethanol into acetylaldehyde. But it can act on a variety of primary unbranched aliphatic alcohol such as 2-ketoisocaproate. We expressed ADH2 in E. coli to use it as part of our Lure Module. Together with kivD it converts 2-ketoisocaproate, which was prepared by LeuABCD, into 3-methyl-1-butanole.2

References

  1. Yeastgenome.org. (2018). ADH2 | SGD. [online] Available at: https://www.yeastgenome.org/locus/S000004918 [Accessed 18 Oct. 2018
  2. Connor, M. R., Cann, A. F. & Liao, J. C. 3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation. Appl. Microbiol. Biotechnol. 86, 1155–1164 (2010).

      3. Sequence and Features


      Assembly Compatibility:
      • 10
        COMPATIBLE WITH RFC[10]
      • 12
        COMPATIBLE WITH RFC[12]
      • 21
        INCOMPATIBLE WITH RFC[21]
        Illegal XhoI site found at 51
      • 23
        COMPATIBLE WITH RFC[23]
      • 25
        INCOMPATIBLE WITH RFC[25]
        Illegal NgoMIV site found at 809
      • 1000
        COMPATIBLE WITH RFC[1000]