Difference between revisions of "Part:BBa K2381012"
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It’s a gRNA sequence containing 20bp targeting at <i>oriC</i> and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.<br/> | It’s a gRNA sequence containing 20bp targeting at <i>oriC</i> and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.<br/> | ||
− | The targeting sequence of this part is acquired from reference paper. | + | The targeting sequence of this part is acquired from reference paper<sup>1</sup>. |
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<img src="https://static.igem.org/mediawiki/parts/d/d2/T--HZAU-China--gRNA_function.png" width="750px"/> | <img src="https://static.igem.org/mediawiki/parts/d/d2/T--HZAU-China--gRNA_function.png" width="750px"/> | ||
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<h3>References</h3> | <h3>References</h3> | ||
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− | Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810. | + | 1. Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810. |
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</body> | </body> |
Latest revision as of 01:00, 18 October 2018
3'-OriC gRNA
It’s a gRNA sequence containing 20bp targeting at oriC and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.
The targeting sequence of this part is acquired from reference paper1.
References
1. Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]