Difference between revisions of "Part:BBa K2560124:Design"

 
 
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===Design Notes===
 
===Design Notes===
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More informations coming soon!
 
  
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This part was created by polymerase chain reaction on the template LVL1 22 + Lux and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly.
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Part 1:
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<b> Forward oligo:</b>
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AACGTCTCGCTCGGGAGGTCTAGGGCGGCGGATTTG
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<b> Reverse Oligo:</b>
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AACGTCTCGTGTCACTGGTGAAAAGAAAAACCAC
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Part 2:
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<b> Forward oligo:</b>
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AACGTCTCGGACACGGGCAACAGCTG
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<b> Reverse Oligo:</b>
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TTCGTCTCCCTCAAGTAGGTCAGTGCGTCCTGCTG
  
  
 
===Source===
 
===Source===
  
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More informations coming soon!
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As source DNA we used LVL1 22 + Lux from the Fritz Lab in Marburg, Germany.
  
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===References===
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Latest revision as of 00:47, 18 October 2018


Phytobrick version of pTrc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created by polymerase chain reaction on the template LVL1 22 + Lux and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.

Part 1:

Forward oligo: AACGTCTCGCTCGGGAGGTCTAGGGCGGCGGATTTG

Reverse Oligo: AACGTCTCGTGTCACTGGTGAAAAGAAAAACCAC


Part 2:

Forward oligo: AACGTCTCGGACACGGGCAACAGCTG

Reverse Oligo: TTCGTCTCCCTCAAGTAGGTCAGTGCGTCCTGCTG


Source

As source DNA we used LVL1 22 + Lux from the Fritz Lab in Marburg, Germany.