Difference between revisions of "Part:BBa K2758000"
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The Killer Red Construct is a double condition suicide sequence. The first condition is the presence of mercury. When the bacteria are in an environment containing mercury, the production of RBS (BBa_B0030) is initiated through the Promotor (BBa_K346002). Then, the Killer Red protein (BBa_K1184000) is produced. The second condition is the presence of light. In order for the Killer red to activate, light (540-585 nm) needs to be shined onto the bacteria to initiate the reaction.
 
The Killer Red Construct is a double condition suicide sequence. The first condition is the presence of mercury. When the bacteria are in an environment containing mercury, the production of RBS (BBa_B0030) is initiated through the Promotor (BBa_K346002). Then, the Killer Red protein (BBa_K1184000) is produced. The second condition is the presence of light. In order for the Killer red to activate, light (540-585 nm) needs to be shined onto the bacteria to initiate the reaction.
  
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===Usage and Biology===
 
===Usage and Biology===
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This part is designed to work in conjunction with a mercury accumulator device, specifically part BBa_K1355003 designed by the iGEM14_UFAM_BRAZIL team.
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We chose to use BBa_B0030 as a moderate binding RBS in order to allow a delay in the expression of Killer Red protein in relation to the accumulator.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2758000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2758000 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
<partinfo>BBa_K2758000 parameters</partinfo>
 
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Revision as of 00:44, 18 October 2018


Mercury activated Killer Red Protein suicide switch

The Killer Red Construct is a double condition suicide sequence. The first condition is the presence of mercury. When the bacteria are in an environment containing mercury, the production of RBS (BBa_B0030) is initiated through the Promotor (BBa_K346002). Then, the Killer Red protein (BBa_K1184000) is produced. The second condition is the presence of light. In order for the Killer red to activate, light (540-585 nm) needs to be shined onto the bacteria to initiate the reaction.

Usage and Biology

This part is designed to work in conjunction with a mercury accumulator device, specifically part BBa_K1355003 designed by the iGEM14_UFAM_BRAZIL team.

We chose to use BBa_B0030 as a moderate binding RBS in order to allow a delay in the expression of Killer Red protein in relation to the accumulator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 237
    Illegal BsaI.rc site found at 528