Difference between revisions of "Part:BBa K2560123:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
More informations coming soon!
 
  
 +
 +
 +
<html>
 +
This part was created by polymerase chain reaction on the template LVL1 23 + Lux and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly.
 +
</html>
 +
 +
 +
<b> Forward oligo:</b>
 +
AACGTCTCGCTCGGGAGTTTTGTTATCAATAAAAAAGGCCCCC
 +
 +
<b> Reverse Oligo:</b>
 +
TTCGTCTCCCTCAAGTATTCACTTTTCTCTATCACTGATAGG
  
  
 
===Source===
 
===Source===
  
−
More informations coming soon!
+
<html>
 +
 
 +
As source DNA we used LVL1 23 + Lux from the Fritz Lab in Marburg, Germany.
  
−
===References===
+
</html>

Latest revision as of 00:43, 18 October 2018


Phytobrick version of pTet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created by polymerase chain reaction on the template LVL1 23 + Lux and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.


Forward oligo: AACGTCTCGCTCGGGAGTTTTGTTATCAATAAAAAAGGCCCCC

Reverse Oligo: TTCGTCTCCCTCAAGTATTCACTTTTCTCTATCACTGATAGG


Source

As source DNA we used LVL1 23 + Lux from the Fritz Lab in Marburg, Germany.