Difference between revisions of "Part:BBa K2680050"
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In this circuit, temperature-sensitive CI is expressed by J23107, a medium-weak constitutive promoter. This temperature-sensitive CI then represses the downstream CI promoter. Once the temperature is sufficiently raised, the ts-CI protein denatures, loosing its repression capabilities. Therefore, the CI promoter is freed, allowing for expression of mf-Lon. | In this circuit, temperature-sensitive CI is expressed by J23107, a medium-weak constitutive promoter. This temperature-sensitive CI then represses the downstream CI promoter. Once the temperature is sufficiently raised, the ts-CI protein denatures, loosing its repression capabilities. Therefore, the CI promoter is freed, allowing for expression of mf-Lon. | ||
+ | |||
+ | ===Characterization=== | ||
+ | Mf-Lon is capable of degrading proteins tagged with a specific pdt tag | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 00:33, 18 October 2018
J23107 ts-CI+ R0051 mf-Lon
Design
This part is a j23107 ts-cI repressed mf-Lon. For more information on mf-Lon, and to see the source of the mf-Lon, see BBa_K2333011
Mechanics
In this circuit, temperature-sensitive CI is expressed by J23107, a medium-weak constitutive promoter. This temperature-sensitive CI then represses the downstream CI promoter. Once the temperature is sufficiently raised, the ts-CI protein denatures, loosing its repression capabilities. Therefore, the CI promoter is freed, allowing for expression of mf-Lon.
Characterization
Mf-Lon is capable of degrading proteins tagged with a specific pdt tag
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 51
Illegal NheI site found at 74 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2903
Illegal AgeI site found at 2987
Illegal AgeI site found at 3193
Illegal AgeI site found at 3218 - 1000COMPATIBLE WITH RFC[1000]