Difference between revisions of "Part:BBa K2779003"
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<partinfo>BBa_K2779003 short</partinfo> | <partinfo>BBa_K2779003 short</partinfo> | ||
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This enzyme is part of Team UAlberta's engineered biosynthetic pathway to produce protoporphyrin IX (PPIX). Expression of this gene in pBAD for protein purification provided functional hydroxymethylbilane that was used for the ''in vitro'' production of PPIX from 5-aminolevulinic acid. | This enzyme is part of Team UAlberta's engineered biosynthetic pathway to produce protoporphyrin IX (PPIX). Expression of this gene in pBAD for protein purification provided functional hydroxymethylbilane that was used for the ''in vitro'' production of PPIX from 5-aminolevulinic acid. | ||
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| + | This gene encodes for porphobilinogen deaminase from Escherichia coli strain K12; EC 2.5.1.61 which is a transferase. It catalyzes the 4-porphobilinogen hydroxymethylbilane as shown by the reaction scheme below: | ||
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| + | [[File:T--UAlberta--HemC_Basic_Part.png|500px]] | ||
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Latest revision as of 00:27, 18 October 2018
HemC
This enzyme is part of Team UAlberta's engineered biosynthetic pathway to produce protoporphyrin IX (PPIX). Expression of this gene in pBAD for protein purification provided functional hydroxymethylbilane that was used for the in vitro production of PPIX from 5-aminolevulinic acid.
This gene encodes for porphobilinogen deaminase from Escherichia coli strain K12; EC 2.5.1.61 which is a transferase. It catalyzes the 4-porphobilinogen hydroxymethylbilane as shown by the reaction scheme below:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]