Difference between revisions of "Part:BBa K2560054:Design"

 
 
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===Design Notes===
 
===Design Notes===
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A Cas9 and dCas9 LVL0 part was created as a CDS part in our Marburg Collection. The cas9 sequence was PCR amplified from pCas9CR4
  
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===Source===
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<a href="https://www.nature.com/articles/srep15096.pdf"> <abbr title="The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in <i>Escherichia coli</i>">(Reisch and Prather 2017).</abbr></a>
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To make this sequence compatible with our toolbox and the iGEM registry, we removed a BsmBI and EcoRI recognition site in the parts sequence creating the Cas9 LVL0 part <a href="https://parts.igem.org/Part:BBa_K2560047"> <abbr title="Link to the iGEM part registry">BBa_K2560047</abbr></a>. 
  
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===References===
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Latest revision as of 00:25, 18 October 2018


Phytobrick version of dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1107
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3386
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 4118


Design Notes

A Cas9 and dCas9 LVL0 part was created as a CDS part in our Marburg Collection. The cas9 sequence was PCR amplified from pCas9CR4 (Reisch and Prather 2017). To make this sequence compatible with our toolbox and the iGEM registry, we removed a BsmBI and EcoRI recognition site in the parts sequence creating the Cas9 LVL0 part BBa_K2560047.