Difference between revisions of "Part:BBa K2560131:Design"
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<b> Reverse Oligo:</b>
 
<b> Reverse Oligo:</b>
 
CTCAAGTAAAGGGGCGCCAGGGGCTCC
 
CTCAAGTAAAGGGGCGCCAGGGGCTCC
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===Source===
 
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As Source DNA we used PYTK from the Dueber Toolbox. <a href="https://pubs.acs.org/doi/abs/10.1021/sb500366v"><abbr title="A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly">(Lee <i>et al.</i> 2015.)</abbr>  </a>
 
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===Source===
 
===Source===
 
The promoter dummy was designed with a high GC-content to reduce propability of transcription initiation.
 
The promoter dummy was designed with a high GC-content to reduce propability of transcription initiation.

Latest revision as of 23:59, 17 October 2018


Phytobrick version of Promoter Dummy


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward oligo: CTCGGGAGCCCCTGGCGCCCCTTTACT

Reverse Oligo: CTCAAGTAAAGGGGCGCCAGGGGCTCC

Source

The promoter dummy was designed with a high GC-content to reduce propability of transcription initiation.