Difference between revisions of "Part:BBa K2587010"
 
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<partinfo>BBa_K2587010 short</partinfo>
 
<partinfo>BBa_K2587010 short</partinfo>
  
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<i>trzC</i> is coding for the ammelide aminohydrolase, which cleaves the amino group from ammelide to convert it to cyanuric Acid (cyanurate). It could be used as the third step of the melamin degradation as descriped in Shaw at al., 2016. The sequence is codon optimized for use as a biobrick standard part.
+
<i>trzC</i> encodes the ammelide aminohydrolase, which cleaves the amino group from ammelide to convert it to cyanuric acid (cyanurate). It could be used as the third step of the melamine degradation as descriped in Shaw <i>at al.</i>, 2016<sup>1</sup>. The sequence is codon optimized for <i>Escherichia coli</i>.
  
 
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<p><strong><h4>References</h4></strong></p>
 
<p><strong><h4>References</h4></strong></p>
  
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<p>Shaw, A. Joe, et al. "Metabolic engineering of microbial competitive advantage for industrial fermentation processes." Science 353.6299 (2016): 583-586.<br>
+
<p><strong>(1)</strong> Shaw, A. Joe, et al. "Metabolic engineering of microbial competitive advantage for industrial fermentation processes." Science 353.6299 (2016): 583-586.<br>
 
http://science.sciencemag.org/content/353/6299/583?casa_token=_q3c5eJ3Gv4AAAAA:3HmgrxE_r8XSnR8YUtF3cLZ9I-luClCJEJcwxy6E_J711AgFcrN7i-ozHCnV_HZQmxteX12dXxw5rMc</p>
 
http://science.sciencemag.org/content/353/6299/583?casa_token=_q3c5eJ3Gv4AAAAA:3HmgrxE_r8XSnR8YUtF3cLZ9I-luClCJEJcwxy6E_J711AgFcrN7i-ozHCnV_HZQmxteX12dXxw5rMc</p>

Latest revision as of 23:52, 17 October 2018


trzC_opt

trzC encodes the ammelide aminohydrolase, which cleaves the amino group from ammelide to convert it to cyanuric acid (cyanurate). It could be used as the third step of the melamine degradation as descriped in Shaw at al., 20161. The sequence is codon optimized for Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1287
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1287
    Illegal NotI site found at 2
    Illegal NotI site found at 1294
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1287
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1287
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 25
    Illegal BsaI.rc site found at 1280


References

(1) Shaw, A. Joe, et al. "Metabolic engineering of microbial competitive advantage for industrial fermentation processes." Science 353.6299 (2016): 583-586.
http://science.sciencemag.org/content/353/6299/583?casa_token=_q3c5eJ3Gv4AAAAA:3HmgrxE_r8XSnR8YUtF3cLZ9I-luClCJEJcwxy6E_J711AgFcrN7i-ozHCnV_HZQmxteX12dXxw5rMc