Difference between revisions of "Part:BBa C0161"

Latest revision as of 23:47, 17 October 2018

autoinducer synthetase for AHL (no LVA)

same as C0061 except no LVA tag

Note: In the Spring 2009 Distribution Plate, the insert size for this part is larger than the expected part size, and could not be amplified using PCR primers specific to LuxI (15 February 2010, olhoshing)

Add more about the biology of this part here

Usage and Biology

Group: Valencia_UPV iGEM 2018
Authors: Adrián Requena Gutiérrez, Carolina Ropero

We have created part BBa_K2656013 , which is the Acyl-homoserine-lactone synthase (LuxI) coding sequence BBa_C0161 adapted to the Golden Braid grammar and so used with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/http://2018.igem.org/Team:Valencia_UPV/Design#GB Golden Gate] assembly methods. Moreover, it was codon optimized for E. coli.

It is part of our our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <br>
-We have created part  [https://parts.igem.org/Part:BBa_K2656013 BBa_K2656013] , which is the Acyl-homoserine-lactone synthase (LuxI) coding sequence [https://parts.igem.org/Part:BBa_C0161  BBa_C0161] adapted to the Golden Braid grammar and so used with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 Golden Gate] assembly methods. Moreover, it was codon optimized for ''E. coli''.
+We have created part  [https://parts.igem.org/Part:BBa_K2656013 BBa_K2656013] , which is the Acyl-homoserine-lactone synthase (LuxI) coding sequence [https://parts.igem.org/Part:BBa_C0161  BBa_C0161] adapted to the Golden Braid grammar and so used with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/http://2018.igem.org/Team:Valencia_UPV/Design#GB Golden Gate] assembly methods. Moreover, it was codon optimized for ''E. coli''.
 It is part of our our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection].