Difference between revisions of "Part:BBa K2560304"

Revision as of 23:40, 17 October 2018

3-HP Sensor. This part is a PhytoBrick variant

This part contains the promoter as well as the coding sequence for the activator HdpR in the 5´region of the part. It also contains the promotor P_HdpR which is regulated by HdpR. This promoter is oriented in reverse to the HdpR codon, facing out of the 3`end of the part. When HdpR binds 3-Hydroxypropionate it changes its conformation, enabling it to bind to P_HdpR and activate transcription of the gene controlled by it.

Usage and Biology

Inducer: 3-Hydroxypropionate
3-Hydroxypropionate is harmless. For induction, a concentration between 1 and 20 mM can be used.
The system stems from the S1 safety strain of P. putida KT2440.
We designed this biobrick in order to get a system with a usable readout for the intracellular 3-Hydroxypropionate concentration.
Can with be combined with a several reporter genes. We had the best results using the LUX operon.

Figure 1: HIER TEXT
this is a schematic, illustrating the working mechanism of the 3HPA sensor

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1108
Illegal NotI site found at 279
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 263
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-[[File:T--Marburg--3HPA-Sensor.png|600px|thumb|left|'''Figure 1''': <b> HIER TEXT </b> <br>WEITERER TEXT]]
+[[File:T--Marburg--3HPA-Sensor.png|600px|thumb|left|'''Figure 1''': <b> HIER TEXT </b> <br>this is a schematic, illustrating the working mechanism of the 3HPA sensor]]
 </div>