Difference between revisions of "Part:BBa K2787030"
| Line 5: | Line 5: | ||
An improved version of pT181 sense target with its length shortened from 287bp to 201bp and its repressive efficiency improved from 84%for BBa_ to 98%. The part achieve such efficiency through forming secondary structures that can interact with the pT181 antisense and hinder the transcription of downstream genes. With the absence of pT181 antisense, nevertheless, the expression is basically unaffected. | An improved version of pT181 sense target with its length shortened from 287bp to 201bp and its repressive efficiency improved from 84%for BBa_ to 98%. The part achieve such efficiency through forming secondary structures that can interact with the pT181 antisense and hinder the transcription of downstream genes. With the absence of pT181 antisense, nevertheless, the expression is basically unaffected. | ||
| − | + | ==<b>Usage and Biology</b>== | |
| − | + | [[File:ShanghaiTech2018-pT181-1.png|700px|center]] | |
| + | [[File:ShanghaiTech2018-pT181-2.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-3.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-4.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-5.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-6.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-7.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-8.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-9.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-10.png|700px|center]] | ||
<!-- --> | <!-- --> | ||
Revision as of 23:38, 17 October 2018
pT181 Attenuator target sequence with improved repressive efficiency
An improved version of pT181 sense target with its length shortened from 287bp to 201bp and its repressive efficiency improved from 84%for BBa_ to 98%. The part achieve such efficiency through forming secondary structures that can interact with the pT181 antisense and hinder the transcription of downstream genes. With the absence of pT181 antisense, nevertheless, the expression is basically unaffected.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]