Difference between revisions of "Part:BBa K2787029"

Revision as of 23:37, 17 October 2018

RNA attenuator pT181 with GFP under pT181 target sequence by Kyoto 2013 iGEM Team

Measurement of repression efficiency of pT181 sense target submitted by Kyoto 2013 iGEM team(BBa_K1126003) under the expression kit(BBa_K2787027) we designed to test the functionality of our improved version of pT181 sense target(BBa_K2787030). This part is identical to BBa_K2787027 except for the pT181 sense target is changed to BBa_K1126003 and a RBS(BBa_K1893033).

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 268
Illegal NheI site found at 291
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1247

@@ Line 5: / Line 5: @@
 Measurement of repression efficiency of pT181 sense target submitted by Kyoto 2013 iGEM team(BBa_K1126003) under the expression kit(BBa_K2787027) we designed to test the functionality of our improved version of pT181 sense target(BBa_K2787030). This part is identical to BBa_K2787027 except for the pT181 sense target is changed to BBa_K1126003 and a RBS(BBa_K1893033).
-<!-- Add more about the biology of this part here
+==<b>Usage and Biology</b>==
-===Usage and Biology===
+[[File:ShanghaiTech2018-pT181-1.png|700px|center]]
+[[File:ShanghaiTech2018-pT181-2.png|700px|center]]
+[[File:ShanghaiTech2018-pT181-3.png|700px|center]]
+[[File:ShanghaiTech2018-pT181-4.png|700px|center]]
+[[File:ShanghaiTech2018-pT181-5.png|700px|center]]
+[[File:ShanghaiTech2018-pT181-6.png|700px|center]]
+[[File:ShanghaiTech2018-pT181-7.png|700px|center]]
+[[File:ShanghaiTech2018-pT181-8.png|700px|center]]
+[[File:ShanghaiTech2018-pT181-9.png|700px|center]]
+[[File:ShanghaiTech2018-pT181-10.png|700px|center]]
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