Difference between revisions of "Part:BBa K2765053"

Latest revision as of 23:37, 17 October 2018

Complete roGFP2-Orp1 expression cassette started by the promoter FBA1t.

This part consists of the promoter FBA1p , redox sensor roGFP2-Orp1 and the terminator CYC1t. The promoter FBA1p is an constitutive promoter from Saccharomyces cerevisiae CEN.PK2-1c（see the details in part：BBa_K2765040）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1480
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1792

Revision as of 22:52, 17 October 2018 (view source) NarrrEn (Talk \| contribs) ← Older edit		Latest revision as of 23:37, 17 October 2018 (view source) NarrrEn (Talk \| contribs)
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	This part consists of the promoter FBA1p , redox sensor roGFP2-Orp1 and the terminator CYC1t. The promoter FBA1p is an constitutive promoter from <i>Saccharomyces cerevisiae CEN.PK2-1c</i>（see the details in part：BBa_K2765040）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).		This part consists of the promoter FBA1p , redox sensor roGFP2-Orp1 and the terminator CYC1t. The promoter FBA1p is an constitutive promoter from <i>Saccharomyces cerevisiae CEN.PK2-1c</i>（see the details in part：BBa_K2765040）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).

−	[[Image: T--BIT-China--iGEM2018-PartsOutput-~~FBA1-geneline~~.png \|center\|400px\|]]	+	[[Image: T--BIT-China--iGEM2018-PartsOutput-FBA1geneline.png \|center\|400px\|]]