Difference between revisions of "Part:BBa K2832008"

Revision as of 23:37, 17 October 2018

ZntR Inducible mRFP1 Generator ( ZntR ---> ZntAp ---> mRFP1 )

Mechanosensitive channels open to mechanical force. Sound being the mechanical force can open the channel. Zinc ions, among others, enters the cell. Zinc cations bind to zntR, zinc-dependent transcription factor. Active zntR binds to zntAp. zntAp upregulates mRFP to show correct induction. Natural systems, zntA upregulates zntA, a zinc export system. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it stimulates expression of RFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 13
Illegal AgeI site found at 765
Illegal AgeI site found at 877
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2832008 short</partinfo>
-Mechanosensitive channels open to mechanical force. Sound being the mechanical force can open the channel. Zinc ions, among others, enters the cell. Zinc cations bind to zntR, zinc-dependent transcription factor. Active zntR binds to zntAp. zntAp upregulates mRFP to show correct induction. Natural systems, zntA upregulates zntA, a zinc export system. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it allows for the expression of RFP.
+Mechanosensitive channels open to mechanical force. Sound being the mechanical force can open the channel. Zinc ions, among others, enters the cell. Zinc cations bind to zntR, zinc-dependent transcription factor. Active zntR binds to zntAp. zntAp upregulates mRFP to show correct induction. Natural systems, zntA upregulates zntA, a zinc export system. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it stimulates expression of RFP.
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