Difference between revisions of "Part:BBa K2787028"
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The part carries a GFP reporter under J23100 whose coding sequence is placed seamlessly after pT181 sense target. The GFP reporter should express naturally with no repressive effects. A promoter J23100 - scar - terminator sequence is also included as a control group to the pT181 Antisense. This part is used to indicate the normal expression amount of GFP protein, so that when compared with the results from BBa_K2787027 shows the repressive efficiency of pT181. | The part carries a GFP reporter under J23100 whose coding sequence is placed seamlessly after pT181 sense target. The GFP reporter should express naturally with no repressive effects. A promoter J23100 - scar - terminator sequence is also included as a control group to the pT181 Antisense. This part is used to indicate the normal expression amount of GFP protein, so that when compared with the results from BBa_K2787027 shows the repressive efficiency of pT181. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | [[File:ShanghaiTech2018-pT181-1.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-2.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-3.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-4.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-5.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-6.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-7.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-8.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-9.png|700px|center]] | ||
| + | [[File:ShanghaiTech2018-pT181-10.png|700px|center]] | ||
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Revision as of 23:36, 17 October 2018
GFP under pT181 Attenuator target sequence
The part carries a GFP reporter under J23100 whose coding sequence is placed seamlessly after pT181 sense target. The GFP reporter should express naturally with no repressive effects. A promoter J23100 - scar - terminator sequence is also included as a control group to the pT181 Antisense. This part is used to indicate the normal expression amount of GFP protein, so that when compared with the results from BBa_K2787027 shows the repressive efficiency of pT181.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 177
Illegal NheI site found at 200 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1050