Difference between revisions of "Part:BBa K2587018"

Revision as of 23:36, 17 October 2018

P_rpa_RBS_E_T

This construct contains lysis gene E. E is a lysis gene from the bacteriophage PhiX174. This gene is used in our co-culture to induce self lysis of E. coli under the control of the P_rpa</i> promoter. This promoter is part of the Quorum sensing system of <i>Rhodopseudomonas palustris. Together with the other components of the system, RpaR and RpaI, this promoter induces expression of the lysis gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 93

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2587018 short</partinfo>
-This construct contains lysis gene <i>E</I>. <i>E</I> is a lysis gene from the bacteriophage PhiX174. This gene is used in our co-culture to induce self lysis of <i>E. coli</i> under the control of the <i><sub>rpa</i></sub> promoter. This promoter is part of the Quorum sensing system of <i>Rhodopseudomonas palustris</i>. Together with the other components of the system, RpaR and RpaI, this promoter induces expression of the lysis gene.
+This construct contains lysis gene <i>E</I>. <i>E</I> is a lysis gene from the bacteriophage PhiX174. This gene is used in our co-culture to induce self lysis of <i>E. coli</i> under the control of the P<i><sub>rpa</i></sub> promoter. This promoter is part of the Quorum sensing system of <i>Rhodopseudomonas palustris</i>. Together with the other components of the system, RpaR and RpaI, this promoter induces expression of the lysis gene.
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