Difference between revisions of "Part:BBa K2787027"

 
Line 5: Line 5:
 
pT181 is a RNA switch that can repress the expression of target gene by binding its Antisense RNA to its sense target DNA placed upstream of the coding sequenced of target gene. Here the part carries a pT181 Antisense under constitutive promoter J23100 and a GFP reporter whose coding sequence is placed seamlessly after pT181 sense target, also under J23100. This part is used for testing the repressive efficiency of pT181 efficiency by contrast to the results from part BBa_K2787028.
 
pT181 is a RNA switch that can repress the expression of target gene by binding its Antisense RNA to its sense target DNA placed upstream of the coding sequenced of target gene. Here the part carries a pT181 Antisense under constitutive promoter J23100 and a GFP reporter whose coding sequence is placed seamlessly after pT181 sense target, also under J23100. This part is used for testing the repressive efficiency of pT181 efficiency by contrast to the results from part BBa_K2787028.
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
 +
===Usage and Biology===
 +
[[File:ShanghaiTech2018-pT181-1.png|700px|center]]
 +
[[File:ShanghaiTech2018-pT181-2.png|700px|center]]
 +
[[File:ShanghaiTech2018-pT181-3.png|700px|center]]
 +
[[File:ShanghaiTech2018-pT181-4.png|700px|center]]
 +
[[File:ShanghaiTech2018-pT181-5.png|700px|center]]
 +
[[File:ShanghaiTech2018-pT181-6.png|700px|center]]
 +
[[File:ShanghaiTech2018-pT181-7.png|700px|center]]
 +
[[File:ShanghaiTech2018-pT181-8.png|700px|center]]
 +
[[File:ShanghaiTech2018-pT181-9.png|700px|center]]
 +
[[File:ShanghaiTech2018-pT181-10.png|700px|center]]
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 23:34, 17 October 2018


RNA attenuator pT181 with GFP under pT181 target sequence

pT181 is a RNA switch that can repress the expression of target gene by binding its Antisense RNA to its sense target DNA placed upstream of the coding sequenced of target gene. Here the part carries a pT181 Antisense under constitutive promoter J23100 and a GFP reporter whose coding sequence is placed seamlessly after pT181 sense target, also under J23100. This part is used for testing the repressive efficiency of pT181 efficiency by contrast to the results from part BBa_K2787028.


Usage and Biology

ShanghaiTech2018-pT181-1.png
ShanghaiTech2018-pT181-2.png
ShanghaiTech2018-pT181-3.png
ShanghaiTech2018-pT181-4.png
ShanghaiTech2018-pT181-5.png
ShanghaiTech2018-pT181-6.png
ShanghaiTech2018-pT181-7.png
ShanghaiTech2018-pT181-8.png
ShanghaiTech2018-pT181-9.png
ShanghaiTech2018-pT181-10.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 268
    Illegal NheI site found at 291
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1141