Difference between revisions of "Part:BBa K2610030"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. For this, | + | iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. For this, we have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. |
We fused this promotor to green fluorescent protein (see part [https://parts.igem.org/Part:BBa_K2610031 BBa_K2610031]) and stressed transformed DH5a cells for several hours. | We fused this promotor to green fluorescent protein (see part [https://parts.igem.org/Part:BBa_K2610031 BBa_K2610031]) and stressed transformed DH5a cells for several hours. | ||
Latest revision as of 23:30, 17 October 2018
pSoxS
Regulatory protein SoxS is involved in the oxidative stress signaling pathway in Escherichia coli. Intracellular superoxide-generating compounds cause the SoxR to activate transcription of SoxS, which then triggers a set of defense and repair genes that form the oxidative response system.
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. For this, we have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused this promotor to green fluorescent protein (see part BBa_K2610031) and stressed transformed DH5a cells for several hours.
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the SoxS promoter is specifically activated by nalidixic acid and hydrogen peroxide, which both cause oxidative stress.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]