Difference between revisions of "Part:BBa K2765052"

Revision as of 23:26, 17 October 2018

Complete roGFP2-Orp1 expression cassette started by the promoter ENO2.

This part consists of the promoter ENO2p , redox sensor roGFP2-Orp1 and the terminator cyc1. The promoter ENO2p is an constitutive promoter from Saccharomyces cerevisiae CEN.PK2-1c（see the details in part：BBa_K2765042）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).

T--BIT-China--iGEM2018-PartsOutput-ENO2-protein.1.png

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1361
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1673

Revision as of 21:42, 17 October 2018 (view source) Kpcofgs (Talk \| contribs) ← Older edit		Revision as of 23:26, 17 October 2018 (view source) NarrrEn (Talk \| contribs) Newer edit →
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	This part consists of the promoter ENO2p , redox sensor roGFP2-Orp1 and the terminator cyc1. The promoter ENO2p is an constitutive promoter from <i>Saccharomyces cerevisiae CEN.PK2-1c</i>（see the details in part：BBa_K2765042）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).		This part consists of the promoter ENO2p , redox sensor roGFP2-Orp1 and the terminator cyc1. The promoter ENO2p is an constitutive promoter from <i>Saccharomyces cerevisiae CEN.PK2-1c</i>（see the details in part：BBa_K2765042）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).

−	[[Image: T--BIT-China--iGEM2018-PartsOutput-~~ENO2geneline~~.png \|center\|400px\|]]	+	[[Image: T--BIT-China--iGEM2018-PartsOutput-ENO2-protein.1.png \|center\|400px\|]]