Difference between revisions of "Part:BBa K2859001:Experience"
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| + | We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel. | ||
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| + | https://res.cloudinary.com/dt6jjveot/image/upload/SDS.jpg | ||
Revision as of 23:22, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2859001
User Reviews
UNIQefe8eecba521cf74-partinfo-00000000-QINU UNIQefe8eecba521cf74-partinfo-00000001-QINU
We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.
