Difference between revisions of "Part:BBa K914000"
 
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This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here).
 
This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here).
 
This part is used to allow the expression of gene that have one or many amber codon instead of a Serine.
 
This part is used to allow the expression of gene that have one or many amber codon instead of a Serine.
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This part has been improved by part BBa_K2725016 - supD expressed under J23108 anderson promotor (strength 0.51). This expression level of supD allows the expression of a gene with 5 serine codons substituted for amber STOP codons.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:22, 17 October 2018

pLac-supD-T

This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here). This part is used to allow the expression of gene that have one or many amber codon instead of a Serine.


This part has been improved by part BBa_K2725016 - supD expressed under J23108 anderson promotor (strength 0.51). This expression level of supD allows the expression of a gene with 5 serine codons substituted for amber STOP codons.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 96
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 99