Difference between revisions of "Part:BBa K2675066"

 
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<partinfo>BBa_K2675066 short</partinfo>
 
<partinfo>BBa_K2675066 short</partinfo>
  
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sfGFP expression cassette under the contol of J23110 promoter
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This part is an sfGFP ([[Part:BBa_K2675005|BBa_K2675005]]) expression cassette under the control of the constitutive promoter [[Part:BBa_J23110|BBa_J23110]].
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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In this composite part sfGFP ([[Part:BBa_K2675005|BBa_K2675005]]) was equipped by a custom made RBS ([[Part:BBa_K2675017|BBa_K2675017]]) and placed under the control of the constitutive promoter ([[Part:BBa_J23110|BBa_J23110]]).
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The pictures of ''E. coli'' cells harbouring this part show a very strong sfGFP-LVAtag expression. In contrast, no sfGFP-LVAtag is observed in the absence of the promoter and the RBS ([[Part:BBa_K2675080|BBa_K2675080]]).
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[[File: T--Evry Paris-Saclay--Drops-on-plate BBa K2675080.png|600px]]
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Figure 1. Pictures of ''E. coli'' cells harbouring an empty pSB1C3 backbone, [[Part:BBa_K2675080|BBa_K2675080]] or this part [[Part:BBa_K2675066|BBa_K2675066]].
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We used this part as a positive control of sfGFP expression and as a negative control of promoter regulation.
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Revision as of 23:19, 17 October 2018


sfGFP expression cassette under the control of J23110 promoter

This part is an sfGFP (BBa_K2675005) expression cassette under the control of the constitutive promoter BBa_J23110.

Usage and Biology

In this composite part sfGFP (BBa_K2675005) was equipped by a custom made RBS (BBa_K2675017) and placed under the control of the constitutive promoter (BBa_J23110).

The pictures of E. coli cells harbouring this part show a very strong sfGFP-LVAtag expression. In contrast, no sfGFP-LVAtag is observed in the absence of the promoter and the RBS (BBa_K2675080).

T--Evry Paris-Saclay--Drops-on-plate BBa K2675080.png

Figure 1. Pictures of E. coli cells harbouring an empty pSB1C3 backbone, BBa_K2675080 or this part BBa_K2675066.

We used this part as a positive control of sfGFP expression and as a negative control of promoter regulation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 90
  • 1000
    COMPATIBLE WITH RFC[1000]