Difference between revisions of "Part:BBa K2560130:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | <html> | ||
+ | This part was created by polymerase chain reaction (PCR) on the template <a href="https://parts.igem.org/Part:BBa_K2560128">BBa_K2560128</a> and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. | ||
+ | </html> | ||
+ | <b> Forward oligo:</b> | ||
+ | AACGTCTCGCTCGGCTTTAATTGAAAAAAACTTTGTGATTACCGATC | ||
+ | |||
+ | <b> Reverse Oligo:</b> | ||
+ | TTCGTCTCCCTCATACCCCACATGATCGCTGCCATCC | ||
===Source=== | ===Source=== | ||
− | + | <html> | |
− | = | + | As Source DNA we used <a href="https://parts.igem.org/Part:BBa_K2560128">BBa_K2560128</a> from our Toolbox. |
+ | </html> |
Latest revision as of 23:16, 17 October 2018
Phytobrick version of 5a K660004
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was created by polymerase chain reaction (PCR) on the template BBa_K2560128 and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.
Forward oligo: AACGTCTCGCTCGGCTTTAATTGAAAAAAACTTTGTGATTACCGATC
Reverse Oligo: TTCGTCTCCCTCATACCCCACATGATCGCTGCCATCC
Source
As Source DNA we used BBa_K2560128 from our Toolbox.