Difference between revisions of "Part:BBa K2560128:Design"
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− | As Source DNA we used <a href="https://parts.igem.org/Part:BBa_K660004"> | + | As Source DNA we used <a href="https://parts.igem.org/Part:BBa_K660004">BBa_K660004</a>. |
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Latest revision as of 23:10, 17 October 2018
Phytobrick version of K660004
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was created by polymerase chain reaction (PCR) on the template BBa_K660004 and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.
Forward oligo: AACGTCTCGCTCGAATGATTGAAAAAAACTTTGTGATTACCGATC
Reverse Oligo: TTCGTCTCCCTCAAAGCCACATGATCGCTGCCATCC
Source
As Source DNA we used BBa_K660004.