Difference between revisions of "Part:BBa K2657004"
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<partinfo>BBa_K2657004 short</partinfo>
 
<partinfo>BBa_K2657004 short</partinfo>
 
<h3>RCP+ broad host range promoter (MoClo compatible)</h3>  
 
<h3>RCP+ broad host range promoter (MoClo compatible)</h3>  
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<p>For this improved part, we modified BBa_E1010 and added the synthetic broad host range promoter, CP25, and an RBS sequence to our improved part. The broad host range promoter will be compatible with a higher number of bacterial strains. We also made the sequence functional in MoClo Assembly with BsaI by inserting the sequence into the PhytoBrick universal acceptor, a modified pSB1C3 backbone.</p>  
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<p>For this improved part, we modified [https://parts.igem.org/Part:BBa_E1010 BBa_E1010] and added the synthetic broad host range promoter, CP25, and an RBS sequence to our improved part. The broad host range promoter will be compatible with a higher number of bacterial strains. We also made the sequence functional in MoClo Assembly with BsaI by inserting the sequence into the PhytoBrick universal acceptor, a modified pSB1C3 backbone.</p>  
 
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<img src="https://static.igem.org/mediawiki/2018/7/72/T--Austin_UTexas--KimRCPCP25Plate.png" style="width:400px";>
 
<img src="https://static.igem.org/mediawiki/2018/7/72/T--Austin_UTexas--KimRCPCP25Plate.png" style="width:400px";>
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<figcaption><b>Figure 1.</b> Red circled indicates a red colony, where the RCP was expressed. White circled indicates colonies that did not show up as red.</figcaption>
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<figcaption><b>Figure 1.</b> The red circle indicates a red colony, where the RCP was expressed. The white circles indicate colonies that did not show up as red.</figcaption>
 
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Revision as of 23:09, 17 October 2018


Phytobrick of RCP with an RBS and CP25 (promoter)

RCP+ broad host range promoter (MoClo compatible)

For this improved part, we modified BBa_E1010 and added the synthetic broad host range promoter, CP25, and an RBS sequence to our improved part. The broad host range promoter will be compatible with a higher number of bacterial strains. We also made the sequence functional in MoClo Assembly with BsaI by inserting the sequence into the PhytoBrick universal acceptor, a modified pSB1C3 backbone.

Figure 1. The red circle indicates a red colony, where the RCP was expressed. The white circles indicate colonies that did not show up as red.

Figure 2 The black circle indicates the streaked transformants’ strong red color.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]