Difference between revisions of "Part:BBa K2533047"
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<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
This is one section for lactate utilization part. | This is one section for lactate utilization part. | ||
| − | [[File:T--HUST-China--2018-tonglu-ldha.png |400px|thumb|center|Figure1 | + | [[File:T--HUST-China--2018-tonglu-ldha.png |400px|thumb|center|Figure1. RBS-ldhA]] |
<h2>DNA Gel Electrophoretic</h2> | <h2>DNA Gel Electrophoretic</h2> | ||
To make sure that we get the target gene, we did DNA gel electrophoretic for verification. And here is the result. | To make sure that we get the target gene, we did DNA gel electrophoretic for verification. And here is the result. | ||
| − | [[File:T--HUST-China--2018-jiaotu-ldha and lldp.png|400px|thumb|center|Figure2 | + | [[File:T--HUST-China--2018-jiaotu-ldha and lldp.png|400px|thumb|center|Figure2. Verification of successful transformation of pSB1C3-RBS-ldhA]] |
Our target gene is 1008bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes. | Our target gene is 1008bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes. | ||
<h2>Electrogenesis</h2> | <h2>Electrogenesis</h2> | ||
By detecting the production of lactate after expressing, we might find out whether ldhA could effectively help Rhodopseudomonas palustris produce more lactate. | By detecting the production of lactate after expressing, we might find out whether ldhA could effectively help Rhodopseudomonas palustris produce more lactate. | ||
| − | [[File:T--HUST-China--2018-expression of lactate.png |400px|thumb|center|Figure3 | + | [[File:T--HUST-China--2018-expression of lactate.png |400px|thumb|center|Figure3. shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieves the goal to help strains produce lactate.]] |
It could be demonstrated that the target gene could be expressed in the engineered cells. More lactate has been produced by engineered bacteria. | It could be demonstrated that the target gene could be expressed in the engineered cells. More lactate has been produced by engineered bacteria. | ||
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Latest revision as of 23:05, 17 October 2018
RBS-ldhA
produce lactate
Usage and biology
ldhA refers to fermentative D-lactate dehydrogenase, NAD-dependent, performing the conversion of pyruvate to D-lactate. In this way, Rhodopseudomonas palustris could produce lactate more efficiently, which brings Shewanella more carbon source.
Characterization
This is one section for lactate utilization part.
DNA Gel Electrophoretic
To make sure that we get the target gene, we did DNA gel electrophoretic for verification. And here is the result.
Our target gene is 1008bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.
Electrogenesis
By detecting the production of lactate after expressing, we might find out whether ldhA could effectively help Rhodopseudomonas palustris produce more lactate.
It could be demonstrated that the target gene could be expressed in the engineered cells. More lactate has been produced by engineered bacteria.