Difference between revisions of "Part:BBa K2675195:Design"
 
Line 11: Line 11:
 
===Source===
 
===Source===
  
−
DNA synthesis & Assembly
+
selection from a random sequence library derived by PCR with degenerate primers on [[Part:BBa_K2675057|BBa_K2675057]].
  
 
===References===
 
===References===

Latest revision as of 23:02, 17 October 2018


sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v6) promote


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 80
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 80
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 80
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 80
    Illegal AgeI site found at 227
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_J23110 constitutive promoter + pAimX(full-noTerminator-v6) promoter (BBa_K2675095) + custom made RBS (BBa_K2675016) + sfGFP-LVAtag coding sequence (BBa_K2675006) + the strong L3S2P56 Terminator (BBa_K2675030)

Source

selection from a random sequence library derived by PCR with degenerate primers on BBa_K2675057.

References

[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.

[2] Solovyev V, Salamov A. Automatic Annotation of Microbial Genomes and Metagenomic Sequences. In Metagenomics and its Applications in Agriculture, Biomedicine and Environmental Studies (Ed. R.W. Li), Nova Science Publishers (2011) p. 61-78.

[3] Gautheret D, Lambert A. Direct RNA motif definition and identification from multiple sequence alignments using secondary structure profiles. J Mol Biol (2001) 313, 1003-1011.

[4] Macke T, Ecker D, Gutell R, Gautheret D, Case DA and Sampath R. RNAMotif – A new RNA secondary structure definition and discovery algorithm. Nucleic Acids Res (2001) 29, 4724–4735.

[5] Chen YJ, Liu P, Nielsen AA, Brophy JA, Clancy K, Peterson T, Voigt CA. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods (2013) 10, 659-664.

[6] Herskowitz I, Hagen D. The lysis-lysogeny decision of phage lambda: explicit programming and responsiveness. Annu Rev Genet (1980) 14, 399-445.