Difference between revisions of "Part:BBa K2657003"
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<partinfo>BBa_K2657003 short</partinfo>
 
<partinfo>BBa_K2657003 short</partinfo>
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<p>In the improved BBa_E1010, specific overhangs for MoClo functional elements were added to the flanks of the part to make it into a PhytoBrick. Additionally, BsaI restriction sites were added so that the improved part could be inserted into the Universal Acceptor.</p>
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CDS originally from BBa_E1010. Codes for red chromoprotein that can be used in golden gate reactions. Circled are the colonies that have the correct construct.
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<img src="https://static.igem.org/mediawiki/2018/f/f2/T--Austin_UTexas--rcpphytopp.png" style="width:400px";>
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<img class = "center" src="https://static.igem.org/mediawiki/2018/f/f2/T--Austin_UTexas--rcpphytopp.png" style="width:400px";><figcaption>
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<b>Figure 2</b> White circled indicates colonies of expected phenotype.</figcaption>
 
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Revision as of 22:58, 17 October 2018


Phytobrick of RCP

In the improved BBa_E1010, specific overhangs for MoClo functional elements were added to the flanks of the part to make it into a PhytoBrick. Additionally, BsaI restriction sites were added so that the improved part could be inserted into the Universal Acceptor.

Figure 2 White circled indicates colonies of expected phenotype.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 572
    Illegal AgeI site found at 684
  • 1000
    COMPATIBLE WITH RFC[1000]