Difference between revisions of "Part:BBa K2787017"
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The 16S rRNA C9 under constitutive promoter BBa_J23100(BBa_K2787007) and pCons-oRBSC9-GFP(BBa_K2787002) are linked into one plasmid to justify the proper function of the ribosomal 16SRNA and RBS set C9.  
 
The 16S rRNA C9 under constitutive promoter BBa_J23100(BBa_K2787007) and pCons-oRBSC9-GFP(BBa_K2787002) are linked into one plasmid to justify the proper function of the ribosomal 16SRNA and RBS set C9.  
  
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==<b>Usage and Biology</b>==
 
==<b>Usage and Biology</b>==
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[[File:A2-B8-C9-Orthogonality.png|700px|center]]
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[[File:ShanghaiTech2018-c9 rbs.png|700px|center]]
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[[File:ShanghaiTech2018-C9 16S.png|700px|center]]
  
 
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Revision as of 22:51, 17 October 2018


GFP under C9 orthogonal RBS with orthogonal 16S rRNA C9

The 16S rRNA C9 under constitutive promoter BBa_J23100(BBa_K2787007) and pCons-oRBSC9-GFP(BBa_K2787002) are linked into one plasmid to justify the proper function of the ribosomal 16SRNA and RBS set C9.


Usage and Biology

A2-B8-C9-Orthogonality.png
ShanghaiTech2018-c9 rbs.png
ShanghaiTech2018-C9 16S.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 798
    Illegal NheI site found at 821
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1660
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 707