Difference between revisions of "Part:BBa K2657004"

Revision as of 22:50, 17 October 2018

Phytobrick of RCP with an RBS and CP25 (promoter)

RCP+ broad host range promoter (MoClo compatible)

For this improved part, we modified BBa_E1010 and added the synthetic broad host range promoter, CP25, and an RBS sequence to our improved part. The broad host range promoter will be compatible with a higher number of bacterial strains. We also made the sequence functional in MoClo Assembly with BsaI by inserting the sequence into the PhytoBrick universal acceptor, a modified pSB1C3 backbone.

Figure 1. Red circled indicates a red colony, where the RCP was expressed. White circled indicates colonies that did not show up as red.

Figure 2 The black circle indicates the streaked transformants’ strong red color.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2657004 short</partinfo>
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 <h3>RCP+ broad host range promoter (MoClo compatible)</h3>
 <p>For this improved part, we modified BBa_E1010  and added the synthetic broad host range promoter, CP25, and an RBS sequence to our improved part. The broad host range promoter will be compatible with a higher number of bacterial strains. We also made the sequence functional in MoClo Assembly with BsaI by inserting the sequence into the PhytoBrick universal acceptor, a modified pSB1C3 backbone.</p>