Difference between revisions of "Part:BBa K2787015"
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The 16S rRNA C9 under constitutive promoter BBa_J23100(BBa_K2787007) and pCons-oRBSA2-GFP(BBa_K2787000) are linked into one plasmid to quantify the orthogonality of the C9 and A2 ribosome system.
 
The 16S rRNA C9 under constitutive promoter BBa_J23100(BBa_K2787007) and pCons-oRBSA2-GFP(BBa_K2787000) are linked into one plasmid to quantify the orthogonality of the C9 and A2 ribosome system.
  
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==<b>Usage and Biology</b>==
 
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==<b>Usage and Biology</b>==
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[[File:A2-B8-C9-Orthogonality.png|700px|center]]
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[[File:ShanghaiTech2018-C9 16S.png|700px|center]]
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[[File:ShanghaiTech2018-A2_rbs.png|700px|center]]
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 22:50, 17 October 2018


GFP under A2 orthogonal RBS with orthogonal 16S rRNA C9

The 16S rRNA C9 under constitutive promoter BBa_J23100(BBa_K2787007) and pCons-oRBSA2-GFP(BBa_K2787000) are linked into one plasmid to quantify the orthogonality of the C9 and A2 ribosome system.


Usage and Biology

A2-B8-C9-Orthogonality.png
ShanghaiTech2018-C9 16S.png
ShanghaiTech2018-A2 rbs.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 797
    Illegal NheI site found at 820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1659
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706