Difference between revisions of "Part:BBa K2787013"
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The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the TJU and B8 ribosome system.
 
The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the TJU and B8 ribosome system.
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 22:48, 17 October 2018


GFP under B8 orthogonal RBS with orthogonal 16S rRNA BBa_K2787041

The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the TJU and B8 ribosome system.

Usage and Biology

ShanghaiTech2018-b8 rbs.png
ShanghaiTech-TJU 16S.png
A2-B8-C9-Orthogonality.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 798
    Illegal NheI site found at 821
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1661
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1675
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 707