Difference between revisions of "Part:BBa K2657004"
Line 5: Line 5:
 
<p>For this improved part, we modified BBa_E1010  and added the synthetic broad host range promoter, CP25, and an RBS sequence to our improved part. The broad host range promoter will be compatible with a higher number of bacterial strains. We also made the sequence functional in MoClo Assembly with BsaI by inserting the sequence into the PhytoBrick universal acceptor, a modified pSB1C3 backbone.</p>  
 
<p>For this improved part, we modified BBa_E1010  and added the synthetic broad host range promoter, CP25, and an RBS sequence to our improved part. The broad host range promoter will be compatible with a higher number of bacterial strains. We also made the sequence functional in MoClo Assembly with BsaI by inserting the sequence into the PhytoBrick universal acceptor, a modified pSB1C3 backbone.</p>  
 
<html>
 
<html>
 +
<div class = "floatleft">
 
<img src="https://static.igem.org/mediawiki/2018/7/72/T--Austin_UTexas--KimRCPCP25Plate.png" style="width:400px";>
 
<img src="https://static.igem.org/mediawiki/2018/7/72/T--Austin_UTexas--KimRCPCP25Plate.png" style="width:400px";>
 
<figcaption><b>Figure 1.</b> Red circled indicates a red colony, where the RCP was expressed. White circled indicates colonies that did not show up as red.</figcaption>
 
<figcaption><b>Figure 1.</b> Red circled indicates a red colony, where the RCP was expressed. White circled indicates colonies that did not show up as red.</figcaption>
 +
</div>
 
</html>
 
</html>
 +
<br>
 +
<html>
 +
<div class = "floatright">
 +
<img src="https://static.igem.org/mediawiki/2018/c/ca/T--Austin_UTexas--KimRCPCP25_2.png" style="width:400px";>
 +
<figcaption><b>Figure 2</b> The black circle indicates the streaked transformants’ strong red color.</figcaption>
 +
</div>
 +
</html>
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:47, 17 October 2018


Phytobrick of RCP with an RBS and CP25 (promoter)

RCP+ broad host range promoter (MoClo compatible)

For this improved part, we modified BBa_E1010 and added the synthetic broad host range promoter, CP25, and an RBS sequence to our improved part. The broad host range promoter will be compatible with a higher number of bacterial strains. We also made the sequence functional in MoClo Assembly with BsaI by inserting the sequence into the PhytoBrick universal acceptor, a modified pSB1C3 backbone.

Figure 1. Red circled indicates a red colony, where the RCP was expressed. White circled indicates colonies that did not show up as red.

Figure 2 The black circle indicates the streaked transformants’ strong red color.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]