Difference between revisions of "Part:BBa K2533014"
 
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<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
 
This is a three section for lactate produce and transfer part.
 
This is a three section for lactate produce and transfer part.
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[[File:T--HUST-China--2018-tonglu-TH-gldA-lldP.png ‎|400px|thumb|center|Figure1:TH-gldA-lldP]]
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[[File:T--HUST-China--2018-tonglu-TH-gldA-lldP.png ‎|400px|thumb|center|Figure1. TH-gldA-lldP]]
  
 
<h2>Metabolic figure</h2>
 
<h2>Metabolic figure</h2>
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[[File:T--HUST-China--2018-metabolic figure1.png|400px|thumb|center|Figure2:Metabolic figure of cyanobacteria with TH-gldA-lldP]]
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[[File:T--HUST-China--2018-metabolic figure1.png|400px|thumb|center|Figure2. Metabolic figure of cyanobacteria with TH-gldA-lldP]]
−
With the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with TH-gldA-lldP absorb CO2 into Calvin cycle and produce NADPH. And then TH can transform NADPH into NADH which will be transformed into NAD+ through gldA. On the other hand, gldA can also turn pyruvate into lactate which will be transferred to the environment with the help of lldP.
+
With the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with TH-gldA-lldP absorb CO<sub>2</sub> into Calvin cycle and produce NADPH. And then TH can transform NADPH into NADH which will be transformed into NAD+ through gldA. On the other hand, gldA can also turn pyruvate into lactate which will be transferred to the environment with the help of lldP.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:43, 17 October 2018


RBS-TH-RBS-gldA-RBS-LLDP

produce and release lactate.

Characterization

This is a three section for lactate produce and transfer part.

Figure1. TH-gldA-lldP

Metabolic figure

Figure2. Metabolic figure of cyanobacteria with TH-gldA-lldP

With the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with TH-gldA-lldP absorb CO2 into Calvin cycle and produce NADPH. And then TH can transform NADPH into NADH which will be transformed into NAD+ through gldA. On the other hand, gldA can also turn pyruvate into lactate which will be transferred to the environment with the help of lldP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 68
    Illegal NgoMIV site found at 569
    Illegal NgoMIV site found at 944
    Illegal NgoMIV site found at 1007
    Illegal NgoMIV site found at 1604
    Illegal NgoMIV site found at 2612
    Illegal NgoMIV site found at 2978
    Illegal AgeI site found at 1616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2240