Difference between revisions of "Part:BBa K2533001"
Line 7: Line 7:
 
<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
 
This is a two section for lactate produce and transfer part.
 
This is a two section for lactate produce and transfer part.
−
[[File:T--HUST-China--2018-tonglu-ldhDC-lldP.png ‎|400px|thumb|center|Figure1:ldhDC-lldP]]
+
[[File:T--HUST-China--2018-tonglu-ldhDC-lldP.png ‎|400px|thumb|center|Figure1. ldhDC-lldP]]
  
 
<h2>DNA Gel Electrophoretic</h2>
 
<h2>DNA Gel Electrophoretic</h2>
 
After we link ldhDC and lldP successfully, we design several tags and then add these tags through PCR with an intention to confirm the expression of ldhDC and lldP. As the figure shows, we get the genetic stripe about 3000bp which means the PCR is successful.
 
After we link ldhDC and lldP successfully, we design several tags and then add these tags through PCR with an intention to confirm the expression of ldhDC and lldP. As the figure shows, we get the genetic stripe about 3000bp which means the PCR is successful.
−
[[File:T--HUST-China--2018-jiaotu-ldhD-ldhDC.jpeg|400px|thumb|center|Figure2:Expression of ldhDC-lldP]]
+
[[File:T--HUST-China--2018-jiaotu-ldhD-ldhDC.jpeg|400px|thumb|center|Figure2. Expression of ldhDC-lldP]]
  
 
<h2>Metabolic figure</h2>
 
<h2>Metabolic figure</h2>
−
[[File:T--HUST-China--2018-metabolic figure1.png|400px|thumb|center|Figure3:Metabolic figure of cyanobacteria with ldhDC-lldP]]
+
[[File:T--HUST-China--2018-metabolic figure1.png|400px|thumb|center|Figure3. Metabolic figure of cyanobacteria with ldhDC-lldP]]
−
With the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with ldhDC-lldP absorb CO2 into Calvin cycle and produce pyruvate. And then ldhDC can transfer pyruvate into lactate which will be transport to the environment by lldP.
+
With the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with ldhDC-lldP absorb CO<sub>2</sub> into Calvin cycle and produce pyruvate. And then ldhDC can transfer pyruvate into lactate which will be transport to the environment by lldP.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:26, 17 October 2018


RBS-ldhDc-RBS-lldP

ldhDC can produce lactate, and lldP can release it from cytoplasm to the environment.

Characterization

This is a two section for lactate produce and transfer part.

Figure1. ldhDC-lldP

DNA Gel Electrophoretic

After we link ldhDC and lldP successfully, we design several tags and then add these tags through PCR with an intention to confirm the expression of ldhDC and lldP. As the figure shows, we get the genetic stripe about 3000bp which means the PCR is successful.

Figure2. Expression of ldhDC-lldP

Metabolic figure

Figure3. Metabolic figure of cyanobacteria with ldhDC-lldP

With the help of light and PSI, Synechocystis sp. PCC 6803(cyanobacteria) with ldhDC-lldP absorb CO2 into Calvin cycle and produce pyruvate. And then ldhDC can transfer pyruvate into lactate which will be transport to the environment by lldP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 780
    Illegal BglII site found at 1325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1461
    Illegal AgeI site found at 1924
    Illegal AgeI site found at 2362
    Illegal AgeI site found at 2521
    Illegal AgeI site found at 2533
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1035