Difference between revisions of "Part:BBa K2787015"
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Revision as of 22:21, 17 October 2018
GFP under A2 orthogonal RBS with orthogonal 16S rRNA C9
The 16S rRNA C9 under constitutive promoter BBa_J23100(BBa_K2787007) and pCons-oRBSA2-GFP(BBa_K2787000) are linked into one plasmid to quantify the orthogonality of the C9 and A2 ribosome system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 797
Illegal NheI site found at 820 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1659
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 706