Difference between revisions of "Part:BBa K2765021:Design"
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To determine whether overexpression of <i>yno1</i> can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned <i>yno1</i> is driven by GAL1 promoter, and thus the target gene is induced by galactose and repressed by glucose. | To determine whether overexpression of <i>yno1</i> can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned <i>yno1</i> is driven by GAL1 promoter, and thus the target gene is induced by galactose and repressed by glucose. | ||
| − | [[Image: T--BIT-China--iGEM2018-Partsregulator-4.png |center| | + | [[Image: T--BIT-China--iGEM2018-Partsregulator-4.png |center|300px|]] |
| − | [[Image: T--BIT-China--iGEM2018-Partsregulator-5.png |center| | + | [[Image: T--BIT-China--iGEM2018-Partsregulator-5.png |center|600px|]] |
| − | [[Image: T--BIT-China--iGEM2018-Partsregulator-6.png |center| | + | [[Image: T--BIT-China--iGEM2018-Partsregulator-6.png |center|600px|]] |
Revision as of 22:20, 17 October 2018
To determine whether overexpression of yno1 can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned yno1 is driven by GAL1 promoter, and thus the target gene is induced by galactose and repressed by glucose.
