Difference between revisions of "Part:BBa K2765021"
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__NOTOC__
 
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<partinfo>BBa_K2765021 short</partinfo>
 
<partinfo>BBa_K2765021 short</partinfo>
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===Usage===
  
 
<i>Yno1</i>(also known as aim14，short for Altered Inheritance rate of Mitochondria or ygl160w) encodes a genuine NADPH oxidase (NOX enzymes), which is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion. Experiments have shown that overexpress <i>yno1</i> can increase the accumulation of intracellular ROS.
 
<i>Yno1</i>(also known as aim14，short for Altered Inheritance rate of Mitochondria or ygl160w) encodes a genuine NADPH oxidase (NOX enzymes), which is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion. Experiments have shown that overexpress <i>yno1</i> can increase the accumulation of intracellular ROS.
  
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https://static.igem.org/mediawiki/2018/c/c8/T--BIT-China--PartRegulator_yno1_Fig1_Position.png
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[[Image: T--BIT-China--iGEM2018-Partsregulator-1.png |center| 400px]]
  
 
reference: https://www.yeastgenome.org/locus/S000003128
 
reference: https://www.yeastgenome.org/locus/S000003128
  
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https://static.igem.org/mediawiki/2018/8/88/T--BIT-China--PartRegulator_yno1_Fig2_Overexpress.png
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===Biology===
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[[Image: T--BIT-China--iGEM2018-Partsregulator-2.png |center|400px|]]
  
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Fig.2 overexpress <i>yno1</i>
 
  
  
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https://static.igem.org/mediawiki/2018/a/ae/T--BIT-China--PartRegulator_yno1_Fig3_process_of_Electron_transformation.png
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[[Image: T--BIT-China--iGEM2018-Partsregulator-3.png |center|400px|]]
  
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Fig. 3 process of Electron transformation
 
  
 
Overexpression yno1 gene can causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p
 
Overexpression yno1 gene can causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p

Revision as of 22:04, 17 October 2018


yno1

Usage

Yno1(also known as aim14，short for Altered Inheritance rate of Mitochondria or ygl160w) encodes a genuine NADPH oxidase (NOX enzymes), which is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion. Experiments have shown that overexpress yno1 can increase the accumulation of intracellular ROS.

T--BIT-China--iGEM2018-Partsregulator-1.png

reference: https://www.yeastgenome.org/locus/S000003128

Biology

T--BIT-China--iGEM2018-Partsregulator-2.png


T--BIT-China--iGEM2018-Partsregulator-3.png


Overexpression yno1 gene can causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1569