Difference between revisions of "Part:BBa K2587008"
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<partinfo>BBa_K2587008 short</partinfo>
 
<partinfo>BBa_K2587008 short</partinfo>
  
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<i>guaD</i> encodes an enzyme called Guanine Deaminase. It converts guanine to xanthine and ammonia. It can be used as the second step in the degradation of melamine to ammonia, as shown by Shaw <i>et al.</i> 2016<sup>1</sup>. In this pathway, it converts ammeline to ammelide.
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<i>guaD</i> encodes an enzyme called guanine deaminase which converts guanine to xanthine and ammonia. It can be used as the second step in the degradation of melamine to ammonia, as shown by Shaw <i>et al.</i> 2016<sup>1</sup>. In this pathway, it is able to convert ammeline to ammelide.
  
  

Revision as of 21:49, 17 October 2018


guaD

guaD encodes an enzyme called guanine deaminase which converts guanine to xanthine and ammonia. It can be used as the second step in the degradation of melamine to ammonia, as shown by Shaw et al. 20161. In this pathway, it is able to convert ammeline to ammelide.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1371
    Illegal NotI site found at 2
    Illegal NotI site found at 1378
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1371
    Illegal AgeI site found at 840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 25
    Illegal BsaI.rc site found at 1364


References

(1)Shaw, A. Joe, et al. "Metabolic engineering of microbial competitive advantage for industrial fermentation processes." Science 353.6299 (2016): 583-586.
http://science.sciencemag.org/content/353/6299/583?casa_token=_q3c5eJ3Gv4AAAAA:3HmgrxE_r8XSnR8YUtF3cLZ9I-luClCJEJcwxy6E_J711AgFcrN7i-ozHCnV_HZQmxteX12dXxw5rMc