Difference between revisions of "Part:BBa K2534015"
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[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided The SV40 (Simian Virus 40) promoter contains the SV40 enhancer promoter region and origin of replication for high-level expression and replication in cell lines expressing the large T antigen (e.g. COS-7 and 293T cells). They will not replicate episomally in the absence of the SV40 large T antigen. The SV40 promoter is weak in B cells, but SV40 exhibits high activity in T24 and HCV29 human bladder urethelium carcinoma cell lines. | [http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided The SV40 (Simian Virus 40) promoter contains the SV40 enhancer promoter region and origin of replication for high-level expression and replication in cell lines expressing the large T antigen (e.g. COS-7 and 293T cells). They will not replicate episomally in the absence of the SV40 large T antigen. The SV40 promoter is weak in B cells, but SV40 exhibits high activity in T24 and HCV29 human bladder urethelium carcinoma cell lines. | ||
| − | + | [http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's construction into the packaging vector of the lentiviral delivery. | |
For further information about demonstration of our results, please visit our results page | For further information about demonstration of our results, please visit our results page | ||
Latest revision as of 21:40, 17 October 2018
SV40 Promoter
[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided The SV40 (Simian Virus 40) promoter contains the SV40 enhancer promoter region and origin of replication for high-level expression and replication in cell lines expressing the large T antigen (e.g. COS-7 and 293T cells). They will not replicate episomally in the absence of the SV40 large T antigen. The SV40 promoter is weak in B cells, but SV40 exhibits high activity in T24 and HCV29 human bladder urethelium carcinoma cell lines.
[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's construction into the packaging vector of the lentiviral delivery.
For further information about demonstration of our results, please visit our results page http://2018.igem.org/Team:AFCM-Egypt/Demonstrate For further information about design of our parts, please visit our Parts & Design Page http://2018.igem.org/Team:AFCM-Egypt/Parts
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]