Difference between revisions of "Part:BBa K2602021:Experience"
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===Validation of GFP fluorescence=== | ===Validation of GFP fluorescence=== | ||
| − | The experimental setup: Bacteria were grown until an OD600 of 0.2-0.4 in LB-medium and 25 ug/ml chloramphenicol. They | + | The experimental setup: Bacteria were grown until an OD600 of 0.2-0.4 in LB-medium and 25 ug/ml chloramphenicol. They were then placed in an 96-well plate in quadruplicates. The OD was measured at the beginning and at the end of the experimental time. Fluorescence was measured every 15 minutes for 16 hours and the temperature was set to 37 degrees celsius. The values shown in fig. 1 are the fluorescence intensity divided by the start OD. Excitation was measured at 485 nm and emission at 520 nm. |
[[File:T--Linkoping_Sweden--Lund.png|430px|thumb|center|Figure 1. GFP intensity over 16 hours. Error bars represent SD. Total time is 16 hours and fluorescence was measured every 15 minutes.]] | [[File:T--Linkoping_Sweden--Lund.png|430px|thumb|center|Figure 1. GFP intensity over 16 hours. Error bars represent SD. Total time is 16 hours and fluorescence was measured every 15 minutes.]] | ||
Revision as of 21:38, 17 October 2018
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2018 iGEM team Linkoping Sweden
2018 iGEM team Linkoping Sweden validated this part.
Validation of GFP fluorescence
The experimental setup: Bacteria were grown until an OD600 of 0.2-0.4 in LB-medium and 25 ug/ml chloramphenicol. They were then placed in an 96-well plate in quadruplicates. The OD was measured at the beginning and at the end of the experimental time. Fluorescence was measured every 15 minutes for 16 hours and the temperature was set to 37 degrees celsius. The values shown in fig. 1 are the fluorescence intensity divided by the start OD. Excitation was measured at 485 nm and emission at 520 nm.
