Difference between revisions of "Part:BBa K2765026"

Revision as of 21:38, 17 October 2018

GAL1p+yno1+CYC1t

This part consists of the promoter GAL1, redox sensor yno1 and the terminator cyc1. The promoter GAL1 is an constitutive promoter from Saccharomyces cerevisiae CEN.PK2-1c-GAL1p-yno1-YNO1t. yno1（yeast NADPH oxidase 1） is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion encodes a genuine NADPH oxidase (NOX enzymes),(see the details in part:BBA-K2765007).And thus the target gene yno1 is induced by galactose and repressed by glucose.as shown in Fig.1.

T--BIT-China--iGEM2018-Partsregulator-Fig.1-geneline.png

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2452
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 669
Illegal XhoI site found at 2430
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 286
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 2281

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−	This part consists of the promoter GAL1, redox sensor <i>yno1</i> and the terminator cyc1. The promoter GAL1 is an constitutive promoter from <i>Saccharomyces cerevisiae</i> CEN.PK2-1c-GAL1p-yno1-YNO1t. <i>yno1</i>（yeast NADPH oxidase 1） is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion encodes a genuine NADPH oxidase (NOX enzymes),(see the details in part:BBA-K2765007).And thus the target gene <i>yno1</i> is induced by galactose and repressed by glucose.as shown in Fig.1.	+	This part consists of the promoter GAL1, redox sensor <i>yno1</i> and the terminator <i>cyc1</i>. The promoter GAL1 is an constitutive promoter from <i>Saccharomyces cerevisiae</i> CEN.PK2-1c-GAL1p-yno1-YNO1t. <i>yno1</i>（yeast NADPH oxidase 1） is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion encodes a genuine NADPH oxidase (NOX enzymes),(see the details in part:BBA-K2765007).And thus the target gene <i>yno1</i> is induced by galactose and repressed by glucose.as shown in Fig.1.

	[[Image: T--BIT-China--iGEM2018-Partsregulator-Fig.1-geneline.png \|center\|400px\|]]		[[Image: T--BIT-China--iGEM2018-Partsregulator-Fig.1-geneline.png \|center\|400px\|]]