Difference between revisions of "Part:BBa K2534015"
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[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided The SV40 (Simian Virus 40) promoter contains the SV40 enhancer promoter region and origin of replication for high-level expression and replication in cell lines expressing the large T antigen (e.g. COS-7 and 293T cells). They will not replicate episomally in the absence of the SV40 large T antigen. The SV40 promoter is weak in B cells, but SV40 exhibits high activity in T24 and HCV29 human bladder urethelium carcinoma cell lines.
 
[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided The SV40 (Simian Virus 40) promoter contains the SV40 enhancer promoter region and origin of replication for high-level expression and replication in cell lines expressing the large T antigen (e.g. COS-7 and 293T cells). They will not replicate episomally in the absence of the SV40 large T antigen. The SV40 promoter is weak in B cells, but SV40 exhibits high activity in T24 and HCV29 human bladder urethelium carcinoma cell lines.
  
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[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's construction into the packaging vector of the lentiviral delivery.
  
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For further information about demonstration of our results, please visit our results page
 +
http://2018.igem.org/Team:AFCM-Egypt/Demonstrate
 +
For further information about design of our parts, please visit our Parts & Design Page
 +
http://2018.igem.org/Team:AFCM-Egypt/Parts
 +
 +
 +
https://static.igem.org/mediawiki/parts/c/c1/Chsv40pafcm.png
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:34, 17 October 2018


SV40 Promoter

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided The SV40 (Simian Virus 40) promoter contains the SV40 enhancer promoter region and origin of replication for high-level expression and replication in cell lines expressing the large T antigen (e.g. COS-7 and 293T cells). They will not replicate episomally in the absence of the SV40 large T antigen. The SV40 promoter is weak in B cells, but SV40 exhibits high activity in T24 and HCV29 human bladder urethelium carcinoma cell lines. 

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's construction into the packaging vector of the lentiviral delivery.

For further information about demonstration of our results, please visit our results page http://2018.igem.org/Team:AFCM-Egypt/Demonstrate For further information about design of our parts, please visit our Parts & Design Page http://2018.igem.org/Team:AFCM-Egypt/Parts


Chsv40pafcm.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]