Difference between revisions of "Part:BBa K2534015:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2534015 short</partinfo>
 
<partinfo>BBa_K2534015 short</partinfo>
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[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided The SV40 (Simian Virus 40) promoter contains the SV40 enhancer promoter region and origin of replication for high-level expression and replication in cell lines expressing the large T antigen (e.g. COS-7 and 293T cells). They will not replicate episomally in the absence of the SV40 large T antigen. The SV40 promoter is weak in B cells, but SV40 exhibits high activity in T24 and HCV29 human bladder urethelium carcinoma cell lines.
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[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's construction into the packaging vector of the lentiviral delivery.
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For further information about demonstration of our results, please visit our results page
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http://2018.igem.org/Team:AFCM-Egypt/Demonstrate
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For further information about design of our parts, please visit our Parts & Design Page
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http://2018.igem.org/Team:AFCM-Egypt/Parts
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https://static.igem.org/mediawiki/parts/c/c1/Chsv40pafcm.png
  
 
<partinfo>BBa_K2534015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2534015 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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IDT
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This part has been synthesized through IDT  
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===Source===
 
===Source===
  
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IDT
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http://2018.igem.org/Team:AFCM-Egypt/Bioinformatics_Page_link
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===References===
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Latest revision as of 21:33, 17 October 2018


SV40 Promoter

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided The SV40 (Simian Virus 40) promoter contains the SV40 enhancer promoter region and origin of replication for high-level expression and replication in cell lines expressing the large T antigen (e.g. COS-7 and 293T cells). They will not replicate episomally in the absence of the SV40 large T antigen. The SV40 promoter is weak in B cells, but SV40 exhibits high activity in T24 and HCV29 human bladder urethelium carcinoma cell lines. 

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] have characterized this part considering it's construction into the packaging vector of the lentiviral delivery.

For further information about demonstration of our results, please visit our results page http://2018.igem.org/Team:AFCM-Egypt/Demonstrate For further information about design of our parts, please visit our Parts & Design Page http://2018.igem.org/Team:AFCM-Egypt/Parts


Chsv40pafcm.png


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been synthesized through IDT


Source

http://2018.igem.org/Team:AFCM-Egypt/Bioinformatics_Page_link