Difference between revisions of "Part:BBa K2665011:Experience"
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| − | [[File:T--Kyoto--K_con.jpeg| | + | [[File:T--Kyoto--K_con.jpeg|350px]] <br> |
| + | [[File:T--Kyoto--Na con.jpeg|350px]]<br> | ||
| + | The graph shown above is K+ or Na+ concentration in cells of S.cerevisiae ena1-nha1 strain. Each gene in the graph was cloned to yeast vectors, which were introduced to yeasts. The word “high” means high copy plasmid and the word “low” means low copy plasmids. These transformed yeasts are cultured in SD containing 400mM NaCl. Detailed data are on our wiki.'''[http://2018.igem.org/Team:Kyoto Kyoto2018]''' | ||
| + | These results show that mangrin also contributes to accumulation of K+ and Na+ in a yeast cell. | ||
| + | In addition, mangrin on the high copy plasmid inhibit growth of yeasts too extensively to form colonies. | ||
| + | |||
===Applications of BBa_K2665011=== | ===Applications of BBa_K2665011=== | ||
Revision as of 21:31, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Pictures shown above is colonies of S. cerevisiae ena1- strain on SD midium containing 400mM NaCl. In this spot assay, part BBa_K2665011 cloned to a yeast low-copy vector was used. This result shows that mangrin contributes to salt tolerance of yeasts.
The graph shown above is K+ or Na+ concentration in cells of S.cerevisiae ena1-nha1 strain. Each gene in the graph was cloned to yeast vectors, which were introduced to yeasts. The word “high” means high copy plasmid and the word “low” means low copy plasmids. These transformed yeasts are cultured in SD containing 400mM NaCl. Detailed data are on our wiki.[http://2018.igem.org/Team:Kyoto Kyoto2018]
These results show that mangrin also contributes to accumulation of K+ and Na+ in a yeast cell.
In addition, mangrin on the high copy plasmid inhibit growth of yeasts too extensively to form colonies.
Applications of BBa_K2665011
User Reviews
UNIQ55b96d259e802ca6-partinfo-00000000-QINU UNIQ55b96d259e802ca6-partinfo-00000001-QINU