Difference between revisions of "Part:BBa K2765026:Design"

 
 
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===Design Notes===
 
===Design Notes===
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yno1 is cloned from the genome of CEN.PK2-1C,ligased with pESC plasmid,Pgal1+yno1+cyc1t cloned from pESC plasmid.  
+
<i>yno1</i> is cloned from the genome of CEN.PK2-1C,ligased with pESC plasmid,Pgal1+<i>yno1</i>+cyc1t cloned from pESC plasmid.  
  
  

Latest revision as of 21:23, 17 October 2018


GAL1p+yno1+CYC1t


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 669
    Illegal XhoI site found at 2430
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2281


Design Notes

yno1 is cloned from the genome of CEN.PK2-1C,ligased with pESC plasmid,Pgal1+yno1+cyc1t cloned from pESC plasmid.



Source

S. cerevisiae CEN.PK2-1C


References